Generator

Part:BBa_K2796067

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-08)


pGAL1-mRFP-WPRE-SV40 poly(A) signal

The efficiency of GAL1 promoter could be tested by observing the fluorescence intensity of mRFP after adding galactose of different concentrations.WPRE was used to enhances the export of unspliced mRNA from the nucleus into the cytoplasm. Terminators were added to the compound part to avoid interaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1647
    Illegal AgeI site found at 70
    Illegal AgeI site found at 1003
    Illegal AgeI site found at 1115
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None